Additional file 1: Table S1. of Expressions of VEGF-A and VEGFR-2 in placentae from GDM pregnancies

The detailed clinical information of 10 cases for TEM exam from each group. (DOCX 15 kb

GO molecular functional annotations.

<p>Functional relationship presented with GO “trees” for the top 10 (<i>p</i><0.005) GO annotations (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079897#pone-0079897-t003" target="_blank">Table 3</a>) is shown for both up- and down-regulated A vs. B, A vs. C, and A vs. D. The red color labels indicate the top 10 GOs. The yellow colors show GOs with lower <i>p</i> value, which were not presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079897#pone-0079897-t003" target="_blank">Table 3</a> but could be identified from the differentially expressed lncRNAs in PPROM, FT, PRTB, and PROM.</p

PPROM pathways identified from differentially expressed lncRNAs.

<p>A = PPROM, B = FTB, C = PTB, D = PROM.</p

Validation of lncRNAs.

<p>RT-qPCR was applied to validate differentially expressed lncRNAs between the PPROM and PTB. Seven up-regulated lncRNAs (A) and nine down-regulated ones (B) were analyzed. Other than the sequence name, each lncRNA was labeled with its associated gene accession in GenBank for a purpose of easy access to its associated gene. Significant levels were indicated by * (<i>p</i><0.05) and ** (<i>p</i><0.01).</p

Differential expression of lncRNAs in PPROM.

<p>A = PPROM, B = FTB, C = PTB, D = PROM.</p

Metabolic pathways characterized from the lncRNAs differentially expressed in PPROM.

<p>Six groups of pathways (each group has up- and down-regulated) were characterized with KEGG functional analysis. Three p values, the EASE-score, Fisher-Pvalue and Hypergeometric-Pvalue were integrated for the analysis. The bar plot shows the top Enrichment Score [-log10(Pvalue)] value of the significant enrichment pathway. If there were more than 10 pathways whose Enrichment Score is >0.05, only the top 10 pathways are shown here. The higher Enrichment Score indicates the more lncRNA molecules are involved in this pathway.</p

Differentially expressed lncRNA in coding sequences.

<p>A = PPROM, B = FTB, C = PTB, D = PROM.</p

GO molecular function annotations in PPROM.

<p>A = PPROM, B = FTB, C = PTB, D = PROM.</p

Pathway diagrams.

<p>(A) A diagram of actin cytoskeleton pathway showed all lncRNA molecules identified from A vs. C. Red labels are up-regulated and blue labels are down-regulated lncRNAs at <i>p</i><0.01. Pink labels are the lncRNAs up-regulated at <i>p</i><0.05. Green colors are detected by Arraystar microchip without significance at <i>p</i>>0.05. (B) A diagram of smooth muscle contraction pathway showed all lncRNA molecules identified from A vs. D. Red labels are up-regulated and blue labels are down-regulated lncRNAs at <i>p</i><0.01. Pink labels are the lncRNAs up-regulated at <i>p</i><0.05. Green colors are detected by Arraystar microchip without significance at <i>p</i>>0.05.</p